シュ　ハク   Zhu Bo   朱　博    所属   千葉工業大学  先進工学部 生命科学科    職種   准教授
言語種別	英語
発行・発表の年月	2019/05
形態種別	学術雑誌
査読	査読あり
標題	A simple, real-time assay of horseradish peroxidase using biolayer interferometry
執筆形態	共著
掲載誌名	Bioscience, Biotechnology and Biochemistry
掲載区分	国外
巻・号・頁	83(10),pp.1822-1828
著者・共著者	Takaaki Kojima,Ayako Nakane,Bo Zhu,Almasul Alfi,Hideo Nakano
概要	Horseradish peroxidase (HRP) isoenzyme C1a is one of the most widely used enzymes for various analytical methods in bioscience research and medical fields. In these fields, real-time monitoring of HRP activity is highly desirable because the utility of HRP as a reporter enzyme would be expanded. In this study, we developed a simple assay system enabling real-time monitoring of HRP activity by using biolayer interferometry (BLI). The HRP activity was quantitatively detected on a BLI sensor chip by tracing a binding response of tyramide, a substrate of HRP, onto an immobilized protein. This system could be applied to analyses related to oxidase activity, as well as to the functional analysis of recombinant HRP.
DOI	10.1080/09168451.2019.1621156
PMID	31119970